Journal: ACS Chemical Neuroscience
Article Title: Interplay of Neuroinflammation and Gut Microbiota Dysbiosis in Alzheimer’s Disease Using Diffusion Kurtosis Imaging Biomarker in 3 × Tg-AD Mouse Models
doi: 10.1021/acschemneuro.5c00063
Figure Lengend Snippet: Comprehensive experimental design for assessing AD pathology in mice. The timeline illustrates the multistep workflow for analyzing behavioral, imaging, microbiota, cytokine, and neuroinflammatory changes in 6-MO and 12-MO WT and AD mice ( N = 5 per group). On day 1, cognitive performance was evaluated using the T-maze task to assess working memory. On day 2, MRI/DKI was conducted to analyze microstructural brain changes. On day 3, fecal samples were processed with NGS to evaluate gut microbiota composition and diversity. On day 4, ELISA was used to quantify systemic pro-inflammatory cytokines, including IL-1β, IL-6, TNFα, and IFN-γ. Meanwhile, the serum samples were quantified for SCFA levels to assess dysregulated gut metabolism. On day 5, IHC was performed to assess microglial (Iba1) and astrocytic (GFAP) activation in key brain regions such as the mPFC, HIPP, and STR. This comprehensive workflow integrates multiple methodologies to investigate age- and disease-specific changes in AD pathology.
Article Snippet: Cytokine levels (IL-1β, IL-6, IFN-γ, and TNFα) were quantified using a multiplex ELISA kit (MEK1016; Boster, Pleasanton, CA, USA).
Techniques: Imaging, Enzyme-linked Immunosorbent Assay, Activation Assay